Enzymatic synthesis of cholecalciferol glycosides using β-glucosidase from sweet almond
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چکیده
منابع مشابه
Amyloglucosidase from Rhizopus mold and β-glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides
Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from Rhizopus mold and βglucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, pH, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, Dmannose, D-ribose, sucrose, maltose and lactose, bo...
متن کاملDual role of imidazole as activator/inhibitor of sweet almond (Prunus dulcis) β-glucosidase
The activity of Prunus dulcis (sweet almond) β-glucosidase at the expense of p-nitrophenyl-β-d-glucopyranoside at pH 6 was determined, both under steady-state and pre-steady-state conditions. Using crude enzyme preparations, competitive inhibition by 1-5 mM imidazole was observed under both kinetic conditions tested. However, when imidazole was added to reaction mixtures at 0.125-0.250 mM, we d...
متن کاملSynthesis of Octyl-β-Glucoside Catalyzed by Almond β-Glucosidase in Unconventional Reaction Media.
The reaction system for octyl-β-glucoside synthesis catalysed by the almond-β-glucosidase has been characterised. The monophasic octanol saturated with different amounts of water served as a reaction medium. Both the glucose and the activated substrate p-nitrophenyl-β-glucoside were examined as glycon donors. The reverse hydrolysis and the transglycosylation were both used as reaction models fo...
متن کاملEnzymatic synthesis of glycosides: from natural O- and N-glycosides to rare C- and S-glycosides
Carbohydrate related enzymes, like glycosyltransferases and glycoside hydrolases, are nowadays more easily accessible and are thought to represent powerful and greener alternatives to conventional chemical glycosylation procedures. The knowledge of their corresponding mechanisms has already allowed the development of efficient biocatalysed syntheses of complex O-glycosides. These enzymes can al...
متن کاملsynthesis of amido alkylnaphthols using nano-magnetic particles and surfactants
we used dbsa and nano-magnetic for the synthesis of amido alkylnaphtols.
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ژورنال
عنوان ژورنال: Journal of Food Science and Technology
سال: 2010
ISSN: 0022-1155,0975-8402
DOI: 10.1007/s13197-010-0080-7